Lissamine Green is a synthetic organic dye that belongs to the xanthene family of dyes, and is structurally related to fluorescein and eosin. The dye is identified by its green coloration and is generally available in the form of sodium salt. Lissamine Green is soluble in water, producing a specific green solution, and is mostly used in various staining applications particularly in biological and medical research.
CAS No.: 11096-63-2
Synonyms: Erio green; Lissamina Verde; verde lisamina; Verde di lissamina; Vert Lissamine; Acid green S; Wool green S or C
| Physical Properties | |
| Chemical formula | C₂₇H₂₅N₂O₇S₂Na |
| IUPAC Name | sodium bis4-(dimethylamino)phenylmethylium |
| Molecular weight | 702.3 g/mol |
| Solubility | H2O: 0.1% |
| Flash point | 12°C |
| Density | 0.541 g/cm³ |
| Chemical Properties | |
| Colour | Dark Green to Black |
| State | Powder |
| Melting point | 210 °C (410 °F) |
| Loss on Drying (at 135°C) | <20% |
| λmax | 633 nm |
| Pictograms : |  |
| Hazard Statements : | H302: Harmful if swallowed |
| Precautionary statements : | P261: Avoid breathing dust/fume/gas/mist/vapors/spray. |
Lissamine Green is a synthetic organic dye that belongs to the xanthene family of dyes, and is structurally related to fluorescein and eosin. The dye is identified by its green coloration and is generally available in the form of sodium salt. Lissamine Green is soluble in water, producing a specific green solution, and is mostly used in various staining applications particularly in biological and medical research.
CAS No.: 11096-63-2
Synonyms: Erio green; Lissamina Verde; verde lisamina; Verde di lissamina; Vert Lissamine; Acid green S; Wool green S or C
| Physical Properties | |
| Chemical formula | C₂₇H₂₅N₂O₇S₂Na |
| IUPAC Name | sodium bis4-(dimethylamino)phenylmethylium |
| Molecular weight | 702.3 g/mol |
| Solubility | H2O: 0.1% |
| Flash point | 12°C |
| Density | 0.541 g/cm³ |
| Chemical Properties | |
| Colour | Dark Green to Black |
| State | Powder |
| Melting point | 210 °C (410 °F) |
| Loss on Drying (at 135°C) | <20% |
| λmax | 633 nm |
| Pictograms : | |
| Hazard Statements : | H302: Harmful if swallowed |
| Precautionary statements : | P261: Avoid breathing dust/fume/gas/mist/vapors/spray. |
Lissamine green stains disrupted cell junctions, with peak absorption at 630 nm. A red-free filter reveals stained areas as black, making it ideal for staining the bulbar conjunctiva. standard diagnostic technique for identifying Plasmodium, the parasite that causes malaria. It also serves as the basic stain for classifying lymphomas according to the Kiel classification.
Fluorescein sodium stains less compromised cells, whereas lissamine green targets more compromised cells and areas.
No, lissamine green is not a vital dye, as it stains damaged or dead cells but does not differentiate between living and non-living cells, unlike vital dyes that specifically stain living cells.
Yes, lissamine green can be used to detect corneal epithelial cell degeneration. As it stains the damaged epithelial cells areas.
The main difference between lissamine green and rose bengal is that rose bengal often causes a stinging sensation, while lissamine green is better tolerated with less irritation.
itosis and then staining the condensed chromosomes with Giemsa dye. This process highlights regions rich in Adenine (A) and Thymine (T) base pairs, resulting in distinctive dark bands.
