Rhodamine B which is a fluorescent dye exists in two forms: an “open” fluorescent form in an acidic medium and a “closed” nonfluorescent form in a basic medium. It fluoresces at nearly 610 nm and its fluorescence intensity decreases with an increase in temperature. It is also soluble in water and alcohol, but solutions should be kept in glass as it adsorbs plastics and decomposes in chlorinated water. Its Luminescence quantum yield is 0.65 in basic ethanol, 0.49 in ethanol,1.0 & 0.68 in 94% ethanol.
CAS No.: 81-88-9
Synonyms: Basic Violet 10; Brilliant Pink B; Rhodamine O; Tetraethylrhodamine; Rhodamine 610; C.I. Pigment Violet 1
| Physical Properties | |
| Chemical formula | C28H31ClN2O3 |
| IUPAC Name | 9-(2-carboxyphenyl)-6-(diethylamino)-N,N-diethyl-3H-xanthen-3-iminium chloride |
| Molecular weight | 479.0 g/mol |
| Solubility | Water, Alcohol, Acetic acid, DMSO & Acetone |
| Flash point | 12°C |
| Density | 0.79 g/mL at 20 °C |
| Chemical Properties | |
| Colour | Green |
| State | Solid Powder or Crystals |
| Melting point | 210–211 °C |
| LogP | 1.9-2 |
| pH | 3-4 |
| Pictograms : |   |
| Hazard Statements : | H302: Harmful if swallowed |
| Precautionary statements : | P264: Wash skin thoroughly after handling. |
Rhodamine B which is a fluorescent dye exists in two forms: an “open” fluorescent form in an acidic medium and a “closed” nonfluorescent form in a basic medium. It fluoresces at nearly 610 nm and its fluorescence intensity decreases with an increase in temperature. It is also soluble in water and alcohol, but solutions should be kept in glass as it adsorbs plastics and decomposes in chlorinated water. Its Luminescence quantum yield is 0.65 in basic ethanol, 0.49 in ethanol,1.0 & 0.68 in 94% ethanol.
CAS No.: 81-88-9
Synonyms: Basic Violet 10; Brilliant Pink B; Rhodamine O; Tetraethylrhodamine; Rhodamine 610; C.I. Pigment Violet 1
| Physical Properties | |
| Chemical formula | C28H31ClN2O3 |
| IUPAC Name | 9-(2-carboxyphenyl)-6-(diethylamino)-N,N-diethyl-3H-xanthen-3-iminium chloride |
| Molecular weight | 479.0 g/mol |
| Solubility | Water, Alcohol, Acetic acid, DMSO & Acetone |
| Flash point | 12°C |
| Density | 0.79 g/mL at 20 °C |
| Chemical Properties | |
| Colour | Green |
| State | Solid Powder or Crystals |
| Melting point | 210–211 °C |
| LogP | 1.9-2 |
| pH | 3-4 |
| Pictograms : | |
| Hazard Statements : | H302: Harmful if swallowed |
| Precautionary statements : | P264: Wash skin thoroughly after handling. |
It’s a fluorescence-based application that inhibits mitochondrial function, as it binds to mitochondrial membranes, disrupting the electron transport chain. Further, it acts as a substrate for P-glycoprotein and possibly MRP1, linked to multidrug resistance in cancer cells.
Rhodamine B is widely used in industrial applications, including printing and dyeing in textiles, paper, paints, and leather products.
Rhodamine B is used to track herbicide application and is being tested as a biomarker in oral rabies vaccines to identify animals that have ingested the bait.
Rhodamine dyes can track water flow and movement by measuring how long it takes for the dye to travel between points, aiding in studies of dispersion and pollutant tracing.
