Jenner’s stain is a Romanowsky-type stain that comprises methylene blue and eosin dyes. It stains the acidic and basic components of the cell, especially useful for visualizing cell nuclei and cytoplasmic features. Developed by Leonard Jenner in 1899, it is considered a pioneer of other stains like Wright and Giemsa.
CAS No.: 62851-42-7
Synonyms: Eosinmethylene-blue; Methyl Eosin; C.I.45385
| Physical Properties | |
| Chemical formula | C21H9Br4KO3 |
| IUPAC Name | potassium;2,4,5,7-tetrabromo-9-(2-methoxycarbonyl phenyl)-6-oxo xanthen-3-olate |
| Molecular weight | 700.0g/mol |
| Solubility | Water & Ethanol |
| Flash point | 11.11°C |
| Chemical Properties | |
| Color | Greenish Black |
| State | Powder |
| Melting point | 175–177°C |
| λmax | 540 nm |
| Loss on drying (at 105 °C) | Max. 10.0% |
| Pictograms : | Â  |
| Hazard Statements : | H302+H312: Harmful if swallowed or in contact with skin |
| Precautionary statements : | P264: Wash thoroughly after handling  |
Jenner’s stain is a Romanowsky-type stain that comprises methylene blue and eosin dyes. It stains the acidic and basic components of the cell, especially useful for visualizing cell nuclei and cytoplasmic features. Developed by Leonard Jenner in 1899, it is considered a pioneer of other stains like Wright and Giemsa.
CAS No.: 62851-42-7
Synonyms: Eosinmethylene-blue; Methyl Eosin; C.I.45385
| Physical Properties | |
| Chemical formula | C21H9Br4KO3 |
| IUPAC Name | potassium;2,4,5,7-tetrabromo-9-(2-methoxycarbonyl phenyl)-6-oxo xanthen-3-olate |
| Molecular weight | 700.0g/mol |
| Solubility | Water & Ethanol |
| Flash point | 11.11°C |
| Chemical Properties | |
| Color | Greenish Black |
| State | Powder |
| Melting point | 175–177°C |
| λmax | 540 nm |
| Loss on drying (at 105 °C) | Max. 10.0% |
| Pictograms : | Â |
| Hazard Statements : | H302+H312: Harmful if swallowed or in contact with skin |
| Precautionary statements : | P264: Wash thoroughly after handling  |
The principle of Jenner’s stain is based upon its ability to distinguish stained blood cells by binding to cellular components, particularly nucleic acids, and cytoplasmic elements, which allows for clear differentiation of cell types under a microscope.
Jenner stain is prepared by dissolving Jenner’s dye powder in methanol to create a stock solution. Typically, 0.5g of dye is dissolved in 100 mL of methanol, and the solution can stand for 24 hours before use.
Yes, Jenner’s stain is a type of Romanowsky stain commonly used for staining blood smears and differentiating blood cells.
Yes, Jenner’s stain requires a slightly acidic pH (around pH 6.8) for optimal staining, which helps in achieving proper differentiation of blood cell components.
The shelf life of Jenner’s stain is typically around 2 to 3 years if stored properly in a cool, dry place, away from direct sunlight.
