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ACID FUCHSIN

MacsChem > Biological Stains > ACID FUCHSIN

Acid Fuchsin is an acidic magenta dye, it’s a sodium sulfonate derivative of fuchsin. This dye is broadly used in histology, particularly in staining methods like Masson’s trichrome stain.

CAS No.: 3244-88-0

Synonyms: Acid Violet 19; Fuchsin S; Fuchsin acid; Rubine S; Acid rubin; p-Fuchsine acid; C.I. 42685.

Physical Properties
Chemical formulaC20H17N3Na2O9S3
IUPAC Namedisodium;(3Z)-3-[(4-amino-3-sulfonatophenyl)-(4-amino-3-sulfophenyl)methylidene]-6-imino-5-methylcyclohexa-1,4-diene-1-sulfonate
Molecular weight585.5 g/mol
SolubilityWater, Ethanol & polar solvents
Density1.74 at 20℃
Chemical Properties
ColourDark green
StateCrystalline powder or crystals
Melting point>250°C
λmax546 nm
pH4.0-6.0
  • Histological Staining: Acid fuchsin is often used in histology, particularly in Masson’s trichrome stain, to distinguish muscle tissue from collagen. Muscle tissues stain red, while collagen appears green or blue.
  • Cytoplasmic and Nuclear Staining: Acid fuchsin is used in tissue section staining to color the cytoplasm and nuclei, and helps to differentiate cellular components under a microscope.
  • Collagen Differentiation: Acid fuchsin plays a vital role in distinguishing collagen fibers from other tissue elements, especially in connective tissue studies.
  • Bacterial Identification: Acid fuchsin is employed in microbiology to identify growing bacteria in cultures and samples.
Pictograms :

 

Hazard Statements :

H315: Causes skin irritation
H319: Causes serious eye irritation.
H335: May cause respiratory irritation

Precautionary statements :

P261: Avoid breathing dust/fume/gas/mist/vapors/spray.
P264: Wash hands thoroughly after handling.
P264+P265: Wash hands thoroughly after handling. Do not touch your eyes.
P271: Use only outdoors or in a well-ventilated area.
P280: Wear protective gloves/protective clothing/eye protection/face protection/hearing protection.
P302+P352: IF ON SKIN: wash with plenty of water.
P304+P340: IF INHALED: Remove person to fresh air and keep comfortable for breathing.
P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do – continue rinsing.
P319: Get medical help if you feel unwell.
P332+P317: If skin irritation occurs: Get medical help.
P337+P317: If eye irritation persists: Get medical help.
P362+P364: Take off contaminated clothing and wash it before reuse.
P403+P233: Store in a well-ventilated place. Keep the container tightly closed.
P405: Store locked up.

Acid Fuchsin is an acidic magenta dye, it’s a sodium sulfonate derivative of fuchsin. This dye is broadly used in histology, particularly in staining methods like Masson’s trichrome stain.

CAS No.: 3244-88-0

Synonyms: Acid Violet 19; Fuchsin S; Fuchsin acid; Rubine S; Acid rubin; p-Fuchsine acid; C.I. 42685.

Physical Properties
Chemical formulaC20H17N3Na2O9S3
IUPAC Namedisodium;(3Z)-3-[(4-amino-3-sulfonatophenyl)-(4-amino-3-sulfophenyl)methylidene]-6-imino-5-methylcyclohexa-1,4-diene-1-sulfonate
Molecular weight585.5 g/mol
SolubilityWater, Ethanol & polar solvents
Density1.74 at 20℃
Chemical Properties
ColourDark green
StateCrystalline powder or crystals
Melting point>250°C
λmax546 nm
pH4.0-6.0
  • Histological Staining: Acid fuchsin is often used in histology, particularly in Masson’s trichrome stain, to distinguish muscle tissue from collagen. Muscle tissues stain red, while collagen appears green or blue.
  • Cytoplasmic and Nuclear Staining: Acid fuchsin is used in tissue section staining to color the cytoplasm and nuclei, and helps to differentiate cellular components under a microscope.
  • Collagen Differentiation: Acid fuchsin plays a vital role in distinguishing collagen fibers from other tissue elements, especially in connective tissue studies.
  • Bacterial Identification: Acid fuchsin is employed in microbiology to identify growing bacteria in cultures and samples.
Pictograms :

 

Hazard Statements :

H315: Causes skin irritation
H319: Causes serious eye irritation.
H335: May cause respiratory irritation

Precautionary statements :

P261: Avoid breathing dust/fume/gas/mist/vapors/spray.
P264: Wash hands thoroughly after handling.
P264+P265: Wash hands thoroughly after handling. Do not touch your eyes.
P271: Use only outdoors or in a well-ventilated area.
P280: Wear protective gloves/protective clothing/eye protection/face protection/hearing protection.
P302+P352: IF ON SKIN: wash with plenty of water.
P304+P340: IF INHALED: Remove person to fresh air and keep comfortable for breathing.
P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do – continue rinsing.
P319: Get medical help if you feel unwell.
P332+P317: If skin irritation occurs: Get medical help.
P337+P317: If eye irritation persists: Get medical help.
P362+P364: Take off contaminated clothing and wash it before reuse.
P403+P233: Store in a well-ventilated place. Keep the container tightly closed.
P405: Store locked up.

FAQ's

Acid Fuchsin stain is used in histology to differentiate between muscle and collagen in tissue sections, as well as to identify bacteria in microbiological studies.

Acid fuchsin solution is a dye solution made by dissolving acid fuchsin, an acidic magenta dye, in water or alcohol. It is commonly used in histology and microbiology. The solution is generally used in specific staining protocols, such as Masson’s trichrome stain.

Acid fuchsin can act as a pH indicator, changing color based on the acidity of the solution. It typically transitions from red in acidic conditions to pink in more neutral environments. This property is used in various staining techniques and biochemical assays.

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