Azure A is an organic compound that belongs to the class of thiazine dyes and is identified by its vivid blue color. It is also a component of Giemsa stain.
CAS No.: 531-53-3
Synonyms: Giemsa’s stain; Dimethylthionine; Azure A chloride; Azur A; 3-Amino -7- (dimethylamino) phenothiazin -5-ium chloride; N, N-Dimethylthionine.
Physical Properties | |
Chemical formula | C14H14ClN3S |
IUPAC Name | (7-aminophenothiazin-3-ylidene)-dimethylazanium;chloride |
Molecular weight | 291.8 g/mol |
Solubility | Water, Alcohol & organic solvents |
Flash point | 199.4°C |
Density | 1.78g/cm³ |
Chemical Properties | |
Color | Light Blue to Dark Blue |
State | Crystalline powder or solid. |
Melting point | 290°C |
λmax | 620-634 nm |
pH | 4.0-6.0 |
Pictograms : | ![]() |
Hazard Statements : | H319: Causes serious eye irritation |
Precautionary statements : | P264+P265: Wash hands thoroughly after handling. Do not touch your eyes. P280: Wear protective gloves/protective clothing/eye protection/face protection/hearing protection. P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do – continue rinsing. P337+P317: If eye irritation persists: Get medical help. |
Azure A is an organic compound that belongs to the class of thiazine dyes and is identified by its vivid blue color. It is also a component of Giemsa stain.
CAS No.: 531-53-3
Synonyms: Giemsa’s stain; Dimethylthionine; Azure A chloride; Azur A; 3-Amino -7- (dimethylamino) phenothiazin -5-ium chloride; N, N-Dimethylthionine.
Physical Properties | |
Chemical formula | C14H14ClN3S |
IUPAC Name | (7-aminophenothiazin-3-ylidene)-dimethylazanium;chloride |
Molecular weight | 291.8 g/mol |
Solubility | Water, Alcohol & organic solvents |
Flash point | 199.4°C |
Density | 1.78g/cm³ |
Chemical Properties | |
Color | Light Blue to Dark Blue |
State | Crystalline powder or solid. |
Melting point | 290°C |
λmax | 620-634 nm |
pH | 4.0-6.0 |
Pictograms : | ![]() |
Hazard Statements : | H319: Causes serious eye irritation |
Precautionary statements : | P264+P265: Wash hands thoroughly after handling. Do not touch your eyes. P280: Wear protective gloves/protective clothing/eye protection/face protection/hearing protection. P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do – continue rinsing. P337+P317: If eye irritation persists: Get medical help. |
Azure A dye is used in staining lysosomes in blood smears, detecting mucopolysaccharides, and is commonly used in Giemsa staining.
Azure A is a Basic dye.
Azure A is used in staining procedures to highlight specific cellular components. It stains lysosomes in blood smears and is commonly used in Giemsa stain to differentiate between cellular elements. Azure A binds to acidic components of cells, providing contrast in microscopy.
Azure A is soluble in water and ethanol with solubility levels typically around 10 mg/mL in water.
Yes, Azure A is generally stable under different pH conditions, but its staining properties can vary depending on the pH of the environment. It works best in slightly acidic to neutral pH