Acriflavine Neutral is a topical antiseptic and antimicrobial agent widely used for treating external fungal and bacterial infections in aquarium fish. Unlike its hydrochloride form, Acriflavine Neutral is less irritating and does not affect water pH, making it a preferred choice for delicate aquatic species. Originally developed by Paul Ehrlich in 1912, it has been utilized in medical, research, and industrial applications. Beyond its role in aquatic disease treatment, Acriflavine Neutral is an effective fluorescent dye for labeling high molecular weight RNA and has shown antifungal and antiparasitic properties in cell culture studies. Its versatility extends to treating Velvet disease, Columnaris, Fin & Tail Rot, Mouth Fungus, and protozoan infections in both freshwater and saltwater aquariums, making it a valuable alternative to Malachite Green.
CAS No.:8048-52-0
Synonyms: Acriflavine base; 3,6-Diamino-10-methylacridinium chloride; Euflavine; Neutroflavine; Trypaflavine Neutral.
Resources: Biological Stains | Classification, Examples & Uses
Physical Properties | |
Chemical formula | C27H25ClN6 |
IUPAC Name | acridine-3,6-diamine;10-methylacridin-10-ium-3,6-diamine;chloride |
Molecular weight | 469.0g/mol |
Solubility | Water & Ethanol |
Density | 1.0933 g/cm³ |
Chemical Properties | |
Color | Orange to red or red-brown |
State | Powder |
Melting point | 179-181 °C |
λmax | 457 nm |
Pictograms : | ![]() ![]() |
Hazard Statements : | H302: Harmful if swallowed H315: Causes skin irritation H319: Causes serious eye irritation H335: May cause respiratory irritation H336: May cause drowsiness or dizziness |
Precautionary statements : | P261: Avoid breathing dust/fume/gas/mist/vapors/spray. P280: Wear protective gloves/protective clothing/eye protection/face protection. P301+P312: IF SWALLOWED: call a POISON CENTER/doctor If you feel unwell. P302+P352: IF ON SKIN: wash with plenty of water. P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do – continue rinsing. |
Acriflavine Neutral is a topical antiseptic and antimicrobial agent widely used for treating external fungal and bacterial infections in aquarium fish. Unlike its hydrochloride form, Acriflavine Neutral is less irritating and does not affect water pH, making it a preferred choice for delicate aquatic species. Originally developed by Paul Ehrlich in 1912, it has been utilized in medical, research, and industrial applications. Beyond its role in aquatic disease treatment, Acriflavine Neutral is an effective fluorescent dye for labeling high molecular weight RNA and has shown antifungal and antiparasitic properties in cell culture studies. Its versatility extends to treating Velvet disease, Columnaris, Fin & Tail Rot, Mouth Fungus, and protozoan infections in both freshwater and saltwater aquariums, making it a valuable alternative to Malachite Green.
CAS No.:8048-52-0
Synonyms: Acriflavine base; 3,6-Diamino-10-methylacridinium chloride; Euflavine; Neutroflavine; Trypaflavine Neutral.
Resources: Biological Stains | Classification, Examples & Uses
Physical Properties | |
Chemical formula | C27H25ClN6 |
IUPAC Name | acridine-3,6-diamine;10-methylacridin-10-ium-3,6-diamine;chloride |
Molecular weight | 469.0g/mol |
Solubility | Water & Ethanol |
Density | 1.0933 g/cm³ |
Chemical Properties | |
Color | Orange to red or red-brown |
State | Powder |
Melting point | 179-181 °C |
λmax | 457 nm |
Pictograms : | ![]() ![]() |
Hazard Statements : | H302: Harmful if swallowed H315: Causes skin irritation H319: Causes serious eye irritation H335: May cause respiratory irritation H336: May cause drowsiness or dizziness |
Precautionary statements : | P261: Avoid breathing dust/fume/gas/mist/vapors/spray. P280: Wear protective gloves/protective clothing/eye protection/face protection. P301+P312: IF SWALLOWED: call a POISON CENTER/doctor If you feel unwell. P302+P352: IF ON SKIN: wash with plenty of water. P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do – continue rinsing. |
Acriflavine Neutral is a fluorescent dye used in biological staining and research. It is a derivative of acriflavine, known for its applications in microscopy and as an antiseptic.
Acriflavine neutral in glycerin enhances wound drainage due to its hygroscopic hypertonic action and as a useful antiseptic for wounds.
Acriflavine neutral is applied directly to wounds for antiseptic treatment and used for staining biological samples in laboratory settings. Always follow specific usage instructions and consult a professional if needed.
Yes, Acriflavine Neutral treats fungal infections in fish and aquariums. It is effective against Saprolegnia spp., egg fungus, and other external fungal infections in freshwater and saltwater fish. It is also used as a wound disinfectant to prevent secondary fungal infections.
Acriflavine Neutral may cause skin irritation, allergic reactions, or discoloration of tissues. Prolonged use or high concentrations might increase the risk of these side effects. Always use as directed and consult a healthcare provider if adverse reactions occur.
The pH of the Acriflavine neutral solution is generally 4.5-5.5
It is a mixture of 3,6-diamino-10-methylacridine chloride (trypaflavine) and 3,6-diaminoacridine (proflavine).
Acriflavine Neutral is also known as ‘Euflavine’.