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LISSAMINE GREEN

MacsChem > Biological Stains > LISSAMINE GREEN

Lissamine Green is a synthetic organic dye that belongs to the xanthene family of dyes, and is structurally related to fluorescein and eosin. The dye is identified by its green coloration and is generally available in the form of sodium salt. Lissamine Green is soluble in water, producing a specific green solution, and is mostly used in various staining applications particularly in biological and medical research.

CAS No.: 11096-63-2

Synonyms: Erio green; Lissamina Verde; verde lisamina; Verde di lissamina; Vert Lissamine; Acid green S; Wool green S or C

Physical Properties
Chemical formulaC₂₇H₂₅N₂O₇S₂Na
IUPAC Namesodium bis4-(dimethylamino)phenylmethylium
Molecular weight702.3 g/mol
SolubilityH2O: 0.1%
Flash point12°C
Density0.541 g/cm³
Chemical Properties
ColourDark Green to Black
StatePowder
Melting point210 °C (410 °F)
Loss on Drying (at 135°C)<20%
λmax633 nm
  • Conjunctival Staining: Lissamine green is used in conjunctival staining for detecting early signs of dry eye & irritation in the damaged areas of conjunctiva (the white part of the eye). By highlighting the damaged areas before they show up on the corena. Especially for the contact lens wearers. Lissamine green staining helps to identify dry eyes more speedily & precisely.
  • Corneal staining: Lissamine green corneal staining helps by highlighting damaged areas on the cornea. It is primarily used for identifying severe dry eye conditions, such as Sjögren’s syndrome, where serious corneal damage is present.
  • Elevating Lid Margin: Lissamine green is helpful in detection of lid margin staining as it doesn’t stain the tarsal plate and stays on better during lid inversion. It also improves visibility of Marx’s line, aiding in diagnosing meibomian gland dysfunction.
  • Ophthalmic Strips: Lissamine green ophthalmic strips help detect eye surface abnormalities.This dye stains damaged areas, aiding in diagnosis like dry eye or conjunctivitis.
  •  
Pictograms : 
Hazard Statements :

H302: Harmful if swallowed
H315: Causes skin irritation
H319: Causes serious eye irritation
H335: May cause respiratory irritation

Precautionary statements :

P261: Avoid breathing dust/fume/gas/mist/vapors/spray.
P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do – continue rinsing.

  • Fluorescein: Fluorescein sodium stains less compromised cells, while lissamine green targets more compromised cells and areas. They complement each other by highlighting different aspects of the eye surface.
  • Rose Bengal: Lissamine green is well tolerated and less toxic than rose bengal, giving direct resolution with minimum irritation. Further, it does not affect normal cell viability, contrary to rose bengal, which can be harmful and irritate the eye. Both are effective in evaluating ocular surface conditions.
     

 

 

Lissamine Green is a synthetic organic dye that belongs to the xanthene family of dyes, and is structurally related to fluorescein and eosin. The dye is identified by its green coloration and is generally available in the form of sodium salt. Lissamine Green is soluble in water, producing a specific green solution, and is mostly used in various staining applications particularly in biological and medical research.

CAS No.: 11096-63-2

Synonyms: Erio green; Lissamina Verde; verde lisamina; Verde di lissamina; Vert Lissamine; Acid green S; Wool green S or C

Physical Properties
Chemical formulaC₂₇H₂₅N₂O₇S₂Na
IUPAC Namesodium bis4-(dimethylamino)phenylmethylium
Molecular weight702.3 g/mol
SolubilityH2O: 0.1%
Flash point12°C
Density0.541 g/cm³
Chemical Properties
ColourDark Green to Black
StatePowder
Melting point210 °C (410 °F)
Loss on Drying (at 135°C)<20%
λmax633 nm
  • Conjunctival Staining: Lissamine green is used in conjunctival staining for detecting early signs of dry eye & irritation in the damaged areas of conjunctiva (the white part of the eye). By highlighting the damaged areas before they show up on the corena. Especially for the contact lens wearers. Lissamine green staining helps to identify dry eyes more speedily & precisely.
  • Corneal staining: Lissamine green corneal staining helps by highlighting damaged areas on the cornea. It is primarily used for identifying severe dry eye conditions, such as Sjögren’s syndrome, where serious corneal damage is present.
  • Elevating Lid Margin: Lissamine green is helpful in detection of lid margin staining as it doesn’t stain the tarsal plate and stays on better during lid inversion. It also improves visibility of Marx’s line, aiding in diagnosing meibomian gland dysfunction.
  • Ophthalmic Strips: Lissamine green ophthalmic strips help detect eye surface abnormalities.This dye stains damaged areas, aiding in diagnosis like dry eye or conjunctivitis.
  •  
Pictograms : 
Hazard Statements :

H302: Harmful if swallowed
H315: Causes skin irritation
H319: Causes serious eye irritation
H335: May cause respiratory irritation

Precautionary statements :

P261: Avoid breathing dust/fume/gas/mist/vapors/spray.
P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do – continue rinsing.

  • Fluorescein: Fluorescein sodium stains less compromised cells, while lissamine green targets more compromised cells and areas. They complement each other by highlighting different aspects of the eye surface.
  • Rose Bengal: Lissamine green is well tolerated and less toxic than rose bengal, giving direct resolution with minimum irritation. Further, it does not affect normal cell viability, contrary to rose bengal, which can be harmful and irritate the eye. Both are effective in evaluating ocular surface conditions.
     

 

 

FAQ's

Lissamine green stains disrupted cell junctions, with peak absorption at 630 nm. A red-free filter reveals stained areas as black, making it ideal for staining the bulbar conjunctiva. standard diagnostic technique for identifying Plasmodium, the parasite that causes malaria. It also serves as the basic stain for classifying lymphomas according to the Kiel classification.

Fluorescein sodium stains less compromised cells, whereas lissamine green targets more compromised cells and areas.

No, lissamine green is not a vital dye, as it stains damaged or dead cells but does not differentiate between living and non-living cells, unlike vital dyes that specifically stain living cells.

 Yes, lissamine green can be used to detect corneal epithelial cell degeneration. As it stains the damaged epithelial cells areas.

 The main difference between lissamine green and rose bengal is that rose bengal often causes a stinging sensation, while lissamine green is better tolerated with less irritation.
itosis and then staining the condensed chromosomes with Giemsa dye. This process highlights regions rich in Adenine (A) and Thymine (T) base pairs, resulting in distinctive dark bands.

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